In his heat on Tuesday, Steven Ferguson appeared to have a poor start and finished almost 27 seconds behind the South African winner Alan van Coller. He is also competing in the K2 1000.
In the first heat of the K1 500, Australia's world champion Nathan Baggaley was again beaten by Eirik Veraas Larsen but told Reuters he was confident of beating the Norwegian in the final.
"Eirik is the kind of guy who goes just as quick in the heats as he does in the final so it was going to be tough," he said. "I felt in control of that race... and I felt that I could have responded to it but there was no incentive to do that."
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Baggaley finished behind Larsen in the heat for the K1 1000 on Monday and on Tuesday he was beaten by over a second.
In the women's K2 500, Germany's Birgit Fischer got her campaign for a possible ninth Olympic gold medal underway in fine style, winning the second heat with Carolin Leonhardt by just under a length in the last race of the day.
Joint favourites Hungary won the other heat comfortably.
Fischer is also contesting the K4 500 for an eighth gold and on Monday she qualified directly for the final.
The 42-year-old said she felt as "young as my years".
There was also success for another German, Andreas Dittmer, who qualified for the C1 500 final after qualifying directly on Monday for the C1 1000 final.
Dittmer won gold in the C1 1000 in Sydney.
The second day of the regatta was held in bouncy but fast conditions with several crews struggling to line up straight on the start line.
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