Easter Monday’s early kick off saw Villa secure a club-record 10th-straight win when beating Millwall 1-0 at Villa Park.
Jonathan Kodjia's strike on the half-hour mark was enough to make history for Dean Smith’s side, who sit fifth with two games to go.
Their play-off spot was guaranteed after Bristol City – nine points adrift and with a far inferior goal difference with three games remaining – lost 2-0 at Sheffield Wednesday.
Bristol City’s loss, as well as Middlesbrough’s 3-0 defeat at Nottingham Forest, enabled Derby to move up to sixth and occupy the final play-off spot after a 95th-minute penalty and 101st-minute double from Harry Wilson earned Frank Lampard’s side a 2-0 win over QPR.
Meanwhile, Sheffield United, currently second, boosted their hopes of automatic promotion with a 3-0 win at Hull, with third-placed Leeds suffering a damaging 2-0 defeat at Brentford in Monday's evening match.
Leaders Norwich were held 2-2 at Stoke but remain three points clear of Sheffield United.
Elsewhere, West Brom were held to a 0-0 draw at Reading but are guaranteed a place in the play-offs.
At the other end of the table, Rotherham edged closer to relegation after Birmingham won 3-1 to guarantee their own survival.
Other results saw Blackburn beat already-relegated Bolton 2-0, Swansea win 1-0 at bottom-club Ipswich and Wigan beat Preston 2-0.