Wales have sent home West Brom's Hal Robson-Kanu, Leeds striker Tyler Roberts and Stoke loanee Rabbi Matondo from their squad for breaching team protocols.
Although no details have been given by the Football Association of Wales, Roberts apologised on social media for breaking curfew - although he has since deleted the post.
"Gutted to be leaving the camp early, but rules are rules and I shouldn't have been up in the hotel later than the set time," he wrote.
Ancelotti says Bale deserves positive Real send-off after being named in Wales squad
Sorry to the team, staff and Wales fans. I will continue to work as hard as I can to earn my place in the squad for the Euros.
A statement from the FAW said: "The three players will be returning to their respective clubs this afternoon. The FAW will not be making any further comment."
Meanwhile, Gareth Bale has admitted the current World Cup qualifying campaign may be his final one. If the captain can guide his country to the tournament, he will be 33 by the time it starts in Qatar next year.
- Bale insists he is not being disrespectful to Spurs by discussing Real return
- De Bruyne, Lukaku lead Belgium to comeback win over Wales
"Obviously, there's no hiding we are getting older and possibly it is the last campaign", he said.
"If it is, then it won't change my mindset. I'm going to be 100 per cent committed like always. We still have a big tournament to play in the summer and then continue with these qualifiers after.
"I'm purely focused on the present and what is happening now. Whatever will happen in the future will happen."
Wales lost 3-1 in their first qualifier against Belgium last week but beat Mexico in a friendly over the weekend. They play group leaders Czech Republic in Cardiff on Tuesday.
Brooks shares that he is cancer free and is cleared to continue playing football
World Cup Qualification UEFA
‘They should all be ashamed of themselves’ – Bale hits out at ‘disgusting’ treatment
Share this article