The 23-year-old became embroiled in an expletive-laden rant at umpire Fergus Murphy in his first-round win against Spaniard Roberto Carballes Baena, at one point accusing a line judge of "rigging" his match.
In his second round match later in the day against Canadian Felix Auger-Aliassime, which he lost in three sets, Kyrgios was given another warning by umpire James Keothavong for smashing a ball out of the ground after a call went against him.
Kyrgios, who is never far from controversy wherever he plays, also rowed with fans and a courtside photographer and appeared to be going through the motions in the final games.
Nick Kyrgios of Australia reacts during his mens singles second round match against Felix Auger-Aliassime of Canada during day four of the Fever-Tree Championships at Queens Club on June 20, 2019 in London, United Kingdom.
Image credit: Getty Images
He was fine a total of $7,500 for his conduct in the first match and then another $10,000 for his behaviour in the second.
Afterwards Kyrgios described the performance of the match officials as "a joke" and "horrendous".
"I think it's ridiculous. Like, why not have another umpire ready to come in if that guy's have a terrible day?" Kyrgios said. "I don't understand. I have to pay the fines for it.
"The calls are horrendous, but I get fined and he gets nothing. It's like a black star.